TonB-Dependent Systems of Uropathogenic Escherichia coli : Aerobactin and Heme Transport and TonB Are Required for Virulence in the Mouse

TonB-dependent systems of uropathogenic Escherichia coli: aerobactin and heme transport and TonB are required for virulence in the mouse.

The uropathogenic Escherichia coli strain CFT073 has multiple iron acquisition systems, including heme and siderophore transporters. A tonB mutant derivative of CFT073 failed to use heme as an iron source or to utilize the siderophores enterobactin and aerobactin, indicating that transport of these compounds in CFT073 is TonB dependent. The TonB(-) derivative showed reduced virulence in a mouse...

tonB-independent ferrichrome-mediated iron transport in Escherichia coli spheroplasts.

Although a functional tonB gene product was required for ferrichrome-mediated iron transport in whole cells of Escherichia coli K-12, such transport did not require the tonB+ function in spheroplasts. We suggest that in spheroplasts ferrichrome has direct access to the cytoplasmic membrane and that this is reflected in tonB-independent accumulation of ferrichrome iron. Therefore, the tonB gene ...

Heme Utilization in Vibrio cholerae and Analysis of Domains Involved in the Specificity of TonB for TonB-dependent Receptors

Identification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids.

Sotlillm clodtq~l sul~~t~e/polyacrylarnide gel :tnwlysis ot’ protrins t~codcd by ii sc>rics ot’ to?/ R + plasmids in minicells has icknt~ified the torLB gene product ws a prokin wit)h km apparent, molecular weight of’ 36,000. A parallel analysis of s(‘vcn tort N mnt,ations which have been genetically crosst~i onto a tonB+ plasmid support,s this itkntification; t)he 36,000 M, protein is tlbsent ...

Confined Mobility of TonB and FepA in Escherichia coli Membranes

The important process of nutrient uptake in Escherichia coli, in many cases, involves transit of the nutrient through a class of beta-barrel proteins in the outer membrane known as TonB-dependent transporters (TBDTs) and requires interaction with the inner membrane protein TonB. Here we have imaged the mobility of the ferric enterobactin transporter FepA and TonB by tracking them in the membran...